New era of DNA sequencing and related bioinformatics

9 12 2011

I can say the passed week is a week full of great news.

Wednesday (Nov 30, 2011)
“DNA Sequencing Caught in Deluge of Data” — You must want to read this article.

Monday (Dec 5, 2011)
UK will invest £75M for a new BIOINFORMATICS HUB in current EBI, as part of the ELIXIR project. I guess this must be within the framework of the HORIZON 2020, which the EU’s new programme for research and innovation running from 2014 to 2020 with an €80 billion budget. €80 billion!! OMG!

Tuesday (Dec 6, 2011)
The National Human Genome Research Institute (NHGRI), as part of NIH, announced to invest $416 million on research of using genomic data for medical clinical research, including rare inherit disease. Three genome sequencing centers are funded, including the Broad Institute, Genome Institute at Washington University, and Human Genome Sequencing Center, Baylor College of Medicine.

Whole-genome sequencing seems becoming more and more popular since it’s becoming cheaper and faster. Citing what the ELIXIR website says, “Modern sequencers are capable of analysing the equivalent of a human genome every 14 minutes at a cost of US $5,000.” We cannot imagine how easy to get a personal genome sequenced in next 5 years.

So, next question is: What would you do if it became possible to sequence the equivalent of a full human genome for only $1,000? This is the Question of the Year of Nature in 2007, when the whole-genome sequencing is still quite expensive. Personally I like the Ewan’s answer there: Full employment in bioinformatics. Think about it: if you can get your genome sequence in $1, what would you do to that 3,000,000,000 DNA nucleotides?
First of all, you may want to store it (or maybe not, since it’s too large). There are already companied coming out to store the DNA data, like the startup recently invested by Google Ventures, DNAnexus. But question is: who really wants to store all 3G data file, even though the 3G USB just costs less than $1? To only store the difference (between a particular genome and some reference genome), instead of every bit, might be the final solution.
Next, you want to see/visualize it. “Viewing your genomic data on iPhone” is one of such bleeding topics. I am quite serious, Illumina is working on the project with Apple, and even there are working Apps out already.
The most informative thing next is to analysis the data. It’s also the most challenging one. People including myself are working hard on it (That’s why the Uncle Sam invest money on it). Let’s think about this in a non-academic way. More and more companies smell the big cake (first let’s assume it’s a big cake), for example the genotyping-phenotype company like 23andme, the bioinformatics company like GenomeRequest, and just yesterday I read news about a new company called Spiral Genetics (who collect the open source software to do bioinformatics for customers, in a very smart way). Last week, Nature News blog reports the OpenSNP, a website (maybe a company next month) designed by several undergraduate students from U. Washington, they try to build the website to let people share their personal genotype data (from 23andme, deCODEme etc.). It’s a very cool idea!

OK. I know some people might be still questioning whether we need whole-genome sequencing. For individual research, it’s true that not every case needs whole-genome sequencing. But like from old phone to iphone, or from iphone to iphone3G, 4G, 4Gs… do you really need to change your iphone to Iphone 4Gs? You may not, or I am sure practically you don’t need at all. I am still using my wife’s old Nokia, no touch-screen, but very practical. Then why iphone 4Gs are so popular? Because it’s the trend! The lower cost and human’s desire/curiosity/greediness makes it popular. So the same for personal genome sequencing data. The only thing is to show this in an elegant way that even your grandma can understand. That’s the business!


42 things that change when you have a baby

21 09 2011

What changes when you have a baby? A better question may be: What doesn’t change? Here, writer and mom Rebecca Woolf lists her most notable post-baby observations. Then scroll down to read our favorite comments from readers about how their babies changed their lives.

1. You finally stop to smell the roses, because your baby is in your arms.

2. Where you once believed you were fearless, you now find yourself afraid. [See a reader’s perspective in #22, below.]

3. The sacrifices you thought you made to have a child no longer seem like sacrifices.

4. You respect your body … finally.

5. You respect your parents and love them in a new way.

6. You find that your baby’s pain feels much worse than your own.

7. You believe once again in the things you believed in as a child.

8. You lose touch with the people in your life whom you should have banished years ago.

9. Your heart breaks much more easily.

10. You think of someone else 234,836,178,976 times a day.

11. Every day is a surprise.

12. Bodily functions are no longer repulsive. In fact, they please you. (Hooray for poop!)

13. You look at your baby in the mirror instead of yourself.

14. You become a morning person.

15. Your love becomes limitless, a superhuman power.
Read the rest of this entry »


6 04 2010
哕唆的话,请精简了说 /3
责备的话,请换个方式说 /5
苦话,请裹上“糖衣”说 /6
正话,请从反面去说 /9
深奥的话,请类比着去说 /11
消极的话,请积极地说 /13

欣赏的话,请主动去说 /16
“硬话”,请理直气壮地说 /18
说不清的话,请用事实说 /20
讨价的话,请坚持去说 /22
驳斥的话,请以牙还牙说 /23

鼓励的话,请耐心地说 /26
批评的话,请温和地说 /27
情话,请甜蜜地说 /29
命令之语,要请求着说 /31
宽恕的话,请体谅着说 /34

劝服的话,请委婉地说 /37
伤人的话,请打圆场说 /39
良言忠告,请顺耳去说 /41
露骨的话,请隐藏点说 /43
纠错的话,要留面子说 /45
拒绝的话,请顾及情感说 /47

自嘲的话,请幽默地说 /49
解围的话,请幽默点说 /51
交锋的话,请风趣地说 /53
还击的话,请幽默地说 /54
风趣的话,请分场合说 /56

赞美的话,请真诚地说 /59
动人的话,请真诚地说 /61
道歉的话,请真诚地说 /63
请求之语,请礼貌地说 /65
辩论的话,请用情去说 /67

玩笑的话,请把握分寸说 /70
吹嘘的话,请谦逊地说 /72
多嘴的话,请最好少说 /74
两难的话,请含糊其辞说 /76
诱导的话,请旁敲侧击说 /78

应变的话,请机智地说 /80
说错的话,请借题发挥说 /82
圆谎的话,请巧妙去说 /83

激将的话,请抓逆反心理说 /85
对不礼貌问话,请含沙射影回答 /87

圆场的话,请及时去说 /90
感谢的话,、请快点去说 /92
奉承的话,请把握好时机说 /93
悬念的话,请停顿着说 /95
插嘴的话,请等时机再说 /98

争执的话,请避免去说 /lOl
忌讳的话,请坚决不说 /103
隐私的话,请千万别说 /105
骗人的话,请千万别说 /107
自以为是的话,请切记不说 /109
扫兴的话,请最好不说 /111

难做的事,先从容易处去做 /117
摸准信息的事,有的放矢地去做 /119
繁杂的事,抓住关键点去做 /121
重要的事,放在前面去做 /124
感兴趣的事,要集中精力去做 /127

为老板分忧的事,要主动去做 /130
困难的事,满怀信心去做 /132
认准的事,排除嘲讽去做 /134
出人头地的事,要辛勤去做 /138

今天的事,不要等到明天去做 /140
已经决定的事,快速去做 /142
想好的事,要果断去做 /145
当机立断的事,毫不犹豫地去做 /146
选准目标的事,抓紧时间去做 /148

危急的事,要冷静去做 /151
意外的事,要敏捷去做 /153
碰钉子的事,要绕个弯去做 /155
僵持的事,先退一步再做 /157

晋升的事,要借梯去做 /160
不懂的事,借他人智慧去做 /162
不必亲为的事,放手他人去做 /165

得理的事,不妨让几分去做 /169
不是本职的事,别越位去做 /172
承诺别人的事,要留有余地去做 /174
做不到的事,就退一步不做 /176

过热的事,要凉下来再做 /179
出风头的事,要低调去做 /182
功高盖主的事,要谦让着去做 /185
冒险的事,要谨慎去做 /188
容易冲突的事,平心静气去做 /190

约定俗成的事,不要打破规则去做 /192
别人忽略的事,要钻空子去做 /195
突发事件,要冷静地去做 /197
看不准的事,要静观其变再去做 /200

梦想的事,要有信心去做 /202
绝望的事,不能轻言放弃 /203
吃亏的事,要忍住性子做 /206
利己的事,放长线去做 /208

斤斤计较的事,千万别做 /210
不留余地的事,千万别做 /213
急功近利的事,千万别做 /215
半途而废的事,千万别做 /217


17 10 2008

  从潜在的需求上发现细节 海尔造出可洗地瓜的洗衣机 









  从经济的变化中发现细节 日方在谈判桌上大获全胜 












  从重大的发明和发现中捕捉细节 格兰仕微波炉以低价取胜 








  在平凡事物中创造细节 沃尔玛崛起的奥秘 






















  摘自《细节案例》马金山 马文婷 李玉平著 人民日报出版社2008年4月版29.80元

The 10 Most Dangerous Mistakes YOU Probably Make With Women

17 04 2008


Here Are The Top Ten Reasons Why Men Fail With Women—And How To Make Sure YOU Avoid Every One Of These Deadly Common Mistakes…

-By David DeAngelo, Author Of “Double Your Dating”

MISTAKE #1: Being Too Much Of A “Nice Guy”

Have you ever noticed that the really attractive women never seem to be attracted "nice" guys?

Of course you have.

Just like me, I’m sure you’ve had attractive female friends that always seemed to date "jerks"… but for some reason they were never romantically interested in YOU.

What’s going on here?

It’s actually very simple…

Women don’t base their choices of men on how "nice" a guy is. They choose the men they do because they feel a powerful GUT LEVEL ATTRACTION for them.

And guess what?

Being nice doesn’t make a woman FEEL that powerful ATTRACTION.

And being NICE doesn’t make a woman CHOOSE you.

I realize that this doesn’t make a lot of logical sense, and it’s hard to ACCEPT… but GET OVER IT.

Until you accept this FACT and begin to act on it, you’ll NEVER have the success with women that you want.

MISTAKE #2: Trying To “Convince Her To Like You"

What do most guys do when they meet a woman that they REALLY like… but she’s just not interested?

Right! They try to "convince" the woman to feel differently.


Never, ever, EVER.

You cannot CONVINCE a woman to feel differently about you with "logic and reasoning".

Think about it.

If a woman doesn’t "feel it" for you, how in the world do you expect to change that FEELING by being "reasonable" with her?

But we all do it.

When a woman just isn’t interested, we beg, plead, chase, and do our best to change her mind.

Bad idea. One that will never work.

MISTAKE #3: Looking To Her For Approval Or Permission

In our desire to please women (which we mistakenly think will make them like us), us guys are always doing things to get a woman’s "approval" or "permission".

Another HORRIBLE idea.

Women are NEVER attracted to the types of men who kiss up to them… EVER.

Don’t get me wrong here.

You don’t have to treat women BADLY for them to like you.

But if you think that treating a woman well means "always getting her approval and permission for things", think again.

You will never succeed by looking for approval. Women actually get ANNOYED at men who seek their approval.

Doubt me? Just ask any attractive woman if Wussy guys who chase her around and want her approval annoy her…

MISTAKE #4: Trying To “Buy” Her Affection With Food And Gifts

How many times have you taken a woman out to a nice dinner, bought her gifts and flowers, and had her REJECT you for someone who didn’t treat her even HALF as well as you did?

If you’re like me, then you’ve had it happen a LOT.

Well guess what?

It’s only NATURAL when this happens…

That’s right, I said NATURAL.

When you do these things, you send a clear message:

"I don’t think you’ll like me for who I am, so I’m going to try to buy your attention and affection".

Your good intentions usually come across to women as over-compensation for insecurity, and weak attempts at manipulation. That’s right, I said that women see this as MANIPULATION.

MISTAKE #5: Sharing “How You Feel” Too Early In The Relationship With Her

Another huge and unfortunate mistake that most men make with women is sharing how they "feel" too early on.

Attractive women are rare.

And they get a LOT of attention from men.

Most men don’t realize this, but attractive women are being approached in one way or another ALL THE

An attractive woman is often approached several times a DAY by men who are interested. This translate into dozens of times per week, and often HUNDREDS of times per month.

And guess what?

Attractive women have usually dated a LOT of men.

That’s right. They have EXPERIENCE.

They know what to expect.

And one thing that turns an attractive women off and sends her running away faster than just about anything is a guy who starts saying "You know, I really, REALLY like you" after one or two dates.

This signals to the woman that you’re just like all the other guys who fall for her too fast… and can’t control themselves.

Don’t do it. Lean back. Relax.

There’s a much better way…

MISTAKE #6: Not “Getting” How Attraction Works For Women

Women are VERY different from men when it comes to ATTRACTION.

You need to accept this fact, and deal with it.

When a man sees a beautiful, young, sexy woman, he INSTANTLY feels a sexual attraction.

But does the same apply for women?

Do women feel sexual attraction to men based mostly on looks? Or is something else going on?

Well, after studying this topic for over five full years now, I can tell you that women usually have their "attraction mechanisms" triggered by things OTHER than looks.

Have you ever noticed that you see a lot more average and unattractive men with beautiful women than the other way around?

Think about it.

Women are more attracted to certain qualities in men… and they’re attracted to the way a man makes them FEEL than they are to looks alone.

If you know how to use your body language and communication correctly, you can make women feel the same kind of powerful sexual attraction to you that YOU feel when you see a hot, sexy young woman.

But it’s not an accident. You have to LEARN how to do this.

And ANY guy can learn how…

MISTAKE #7: Thinking That It Takes Money And Looks

One of the most common mistakes that guys make is giving up before they’ve even gotten started… because they think that attractive women are only interested in men who have looks and money… or guys who are a certain height… or guys who are a certain age.

And sure, there are some women who are only interested in these things.

But MOST women are far more interested in a man’s personality than his wallet or his looks.

There are personality traits that attract women like a magnet…

And if you learn what they are and how to use them, YOU can be one of these guys.

YOU DO NOT have to "settle" for a woman just because you aren’t rich, tall, or handsome.

Let me say this again: If you know how to use your body language and communication correctly, you can make women feel the same kind of powerful sexual attraction to you that YOU feel when you see a hot, sexy young woman.

MISTAKE #8: Giving Away All Of Your Power To Women

Earlier I mentioned that it’s a mistake to look to a woman for approval or permission.

Well, another similar tactic that a lot of guys use is GIVING AWAY THEIR POWER to women.

Said differently, guys try to get women to like them by doing whatever the woman wants.

Another bad idea…

Women are NEVER attracted to men that they can walk all over… Women aren’t attracted to Wussies!

MISTAKE #9: Not Knowing EXACTLY What To Do In Each Type Of Situation With Women

Now I’m going to blow your mind…

A woman ALWAYS knows what you’re thinking.

Women are approximately TEN TIMES better than men at reading body language. That’s ten TIMES.

I know, it might be hard to believe. But for example, if you’re out on a date with a woman, and you want to kiss her, she knows it.

And if you don’t know exactly what to do and exactly HOW to kiss her, and you just sit there looking at her and getting nervous, she won’t help!

And this goes for ALL aspects of women and dating…

Approaching a woman, getting her number, asking her out, kissing her, getting physical… everything.

If you don’t know what to do in each situation, you will probably screw it up… and LOSE EVERYTHING.

And you KNOW it.

It is VITALLY important that you know EXACTLY how to go from one step to the next with a woman… from the first meeting, all the way to the bedroom.

MISTAKE #10: Not Getting HELP

This is the biggest mistake of all.

This is the mistake that keeps most men from EVER having the kind of success with women that they truly want.

I know, guys don’t like to make themselves look weak or helpless. We don’t like to ask for help.

Hey, I’ve been there myself.

Let me tell you a little about me and how I figured out how to be successful with women…

About five years ago I became fed up with the fact that I didn’t know how to approach, meet, and get dates with women that I was attracted to.

It frustrated the hell out of me.

One night I was out with a friend, and I saw a woman I wanted to ask out, but I just couldn’t get up the nerve to do it. I can still remember that night… right on the spot I made the decision to do whatever it took to learn how to be successful with women and dating.

Well, after a lot of hard work and trying all kinds of crazy things, I finally figured it all out.

I can now approach just about any woman and get her number almost instantly. I’ve dated models, I’ve dated actresses, and I’ve dated nice, normal, regular girls as well.

It has been a very rewarding experience. I no longer feel that sick, insecure feeling… like I don’t know how to meet women… and I might wind up alone.

I know that anytime, anywhere, I can go out and meet attractive women.

I’ve written a book on the topic, and I’ve done seminars on both coasts of the United States… and taught tens of thousands of men all around the world.

I Now Have A FREE, Three-Times-A-Week Email Newsletter…

…But the REALLY GREAT news is that I now publish a free email newsletter three times a week that teaches any guy how to increase his success with women DRAMATICALLY.

And I’d like to invite you to sign up.

It’s free, there’s no obligation, I’ll never share your email address with anyone, and you can easily remove yourself anytime with no hassles (and no, I’ll never pull any of these tricks where I send you a bunch of unwanted junk email when you try to remove yourself).

Of course, it even get’s better than that…

In addition to my free email newsletter, I also have a killer downloadable eBook that you can download right now and be reading in literally MINUTES from right now.

It’s JAM PACKED with dozens and dozens of specific strategies for overcoming fear, approaching women, getting phone numbers and email address from women quickly, great inexpensive or even free date ideas, and how to take things to a "physical" level smoothly and easily.

To sign up for my free three-times-a-week newsletter AND download your copy of this online eBook, just go here:

Free Newsletter And Download eBook

Oh, And One More Thing…

In this day and age of "instant gratification", I realize that this might just sound like another late-night info-mercial promising to make you rich by next week.

Well, that’s not the case.

I’ve spent a lot of time, effort, and energy writing this eBook. I wanted to design and create a program that ANY guy could easily understand and start using IMMEDIATELY to meet and date more women… without having to lie, do dishonest things, or be "manipulative".

I now believe that ANY man can be more successful with women and dating, and I get emails every day with success stories from guys who are using this program to meet and date wonderful women.

I know, I know… an ebook that can teach a regular guy how to be more successful in the dating world? No way.

Well believe me, this program will DRAMATICALLY increase your success with women… I absolutely guarantee it 100%.

If you’d like to take your success with women and dating to the next level, and have the kind of success that you’ve always wanted, then go sign up for my free newsletter, get all the details, and check out some great free samples of the eBook located here:

Free Newsletter And Download eBook

And I’ll talk to you again soon.

Your Friend,

David DeAngelo

P.S. Do some friends a favor, and FORWARD this article to their email addresses. It might be the biggest gift you ever give them.

Copyright 2008 David DeAngelo Communications Inc., All Rights Reserved. David DeAngelo and Double Your Dating are trademarks of David DeAngelo Communications Inc.



23 02 2008










16 12 2007









——摘自《意林》2007年第11期 文/王焕伟